- 《英漢醫(yī)學詞典》mutation detection
- 突變檢測
下載手機詞典可隨時隨地查詞查翻譯
mutation detection中文什么意思
例句與用法
- mutation detection of abcd1 gene in the molecular diagnosis of x-linked adrenoleukodystrophy by denaturing hplc
法在腎上腺腦白質營養(yǎng)不良分子診斷中的應用 - in our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0.2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0.2m mnng for 2.5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture . we found a 5-fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells . this kind of mutation did not occur immediately after mnng exposure
我們實驗室曾用一特殊的突變檢測系統(tǒng)，直接證明dna損傷劑可在哺乳動物細胞誘發(fā)非定標性突變：首先用低濃度(0.2m)的短壽烷化劑mnng(半壽期為1.1hr)處理細胞2.5h后，繼續(xù)培養(yǎng)24h，將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制，發(fā)現在未受致癌物直接攻擊的穿梭質粒中有較自發(fā)突變率高5倍以上的靶基因突變。